Natural antioxidants such as vitamin C, vitamin E, resveratrol, and coenzyme Q10 help reduce the impact of these agents, promoting cellular health and delaying visible signs of aging, such as fine lines and loss of firmness
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The antibodies (and their dilution) used in this study were: myc (mouse Covance, rabbit Proteintech, 16286-1-AP 1/3000e), histone 3 (Abcam, 1791, 1/20,000e), GFP (Life Technologies, A11122, 1/3,000e), GST (Cell signalling, 2624, 1/5,000e), MBP (Cell signalling, 2396, 1/3,000e), nanobody-alpha-HRP (Nanotag, N1505, 1/2,000e) Protein purification and GST pull-down His-tagged and GST-tagged proteins were cloned for this study or from our previous work 21,35 in pET or pGEX-6P plasmids, respectively, and are listed in the resource table
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However, investigation of the effect of UA on such models is limited by the profound differences between humans and e.g., rodents in UA metabolism, primarily due to the loss in humans of a functional uricase
