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Preparations of standard & placebo sample solutions for HPLC analysis Preparation of NAC & Di-NAC standard solutions A stock solution of NAC and Di-NAC standard for method development was prepared by accurately weighed out 50 mg of NAC and Di-NAC transferred into separate 25 ml of volumetric flasks

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The membranes were subsequently probed with antibodies for UCP1 (662045, Millipore, 1:2000)

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The most common side effects include: Nausea Vomiting Diarrhea Constipation Hypoglycemia (low blood sugar levels) Headache Dizziness Injection Site Reactions Understanding these side effects is the first step toward managing them effectively