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[DOI] [PubMed] [Google Scholar] 78.Hassan MQ, Hadi RA, Al-Rawi ZS, Padron VA, Stohs SJ

Effects of intermittent and chronic calorie restriction on mammalian target of rapamycin (mTOR) and IGF-I signaling pathways in mammary fat pad tissues and mammary tumors

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For colony formation assay, cells were cultured in 24-well tissue culture plates for a week to form colonies before being fixed with 10% neutral formalin, stained with 0.5% crystal violet solution, and having the dye extracted by adding 10% acetic acid

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