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Step 8: Inspect the Solution The reconstituted solution should be: Clear no visible particles, cloudiness, or floating debris Consistent no undissolved powder remaining at the bottom Free of foam if foam is present, let it settle before use If the solution is cloudy, contains particles, or wont dissolve fully, do not use it

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The rats that failed to reach the platform within 60 s were gently directed (without being removed from the water) to the platform and left there for 15 s (the latency period for those mice was recorded as 60 s)

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A standard curve with known GSH concentrations was used to calibrate all the results