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Sequencing was carried out by Sanger methodology on ABI PRISM 310 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA) and 100% concordance with restriction fragment length polymorphism assays was shown providing confidence in using the full dataset in subsequent analysis

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Spironolactone (brand names: Aldactone and CaroSpir) is a potassium-sparing diuretic (water pill) approved by the Food and Drug Administration (FDA) to treat congestive heart failure, high blood pressure (hypertension), edema (fluid retention) associated with liver cirrhosis or certain kidney problems, and hyperaldosteronism, which is an excess of the hormone aldosterone that can cause high blood pressure

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The hormone changes associated with the menopause transition can fundamentally alter how your body processes and stores fat at a cellular level

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