These processes reduce glutathione (GSH) production, linking elevated insulin, glucose, and Hcy to lower GSH levels.44 Thyroid hormones Hyperthyroidism or prolonged synthetic thyroid hormone use affects liver function, with 24% showing altered GGT levels
Western blot analysis RIPA Lysis Buffer (TransGen, Beijing, China) was used to extract total protein from tissues/fibroblasts and a Nuclear and Cytoplasmic Protein Extraction Kit (TransGen) was used to extract the protein from nucleus and cytoplasm of fibroblasts
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