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These changes contribute to neuroinflammation, bloodbrain barrier (BBB) dysfunction, microglial activation, and neurotransmitter imbalances

Furthermore, variability associated with adverse event reporting, the terminology used to describe adverse events, etc., limit the value of the quantitative frequency estimates provided

doi: 10.1371/journal.pone.0048562 131

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DNA constructs and mutagenesis PCR-amplified human SLC2A1 , DHHC9 , and GCP16 were subcloned into baculoviral vector pFastBac1, lentiviral vector pCDH (hygromycin resistance), bicistronic expression vector pIRES, or green fluorescent protein vector pEGFP-C1

Patients often exhibit structurally abnormal, swollen mitochondria with disrupted cristae, impaired mitochondrial recycling (a process called mitophagy), and chronically elevated levels of reactive oxygen species