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HLCs were cultured with Alexa-Flour 488-ac-LDL for 1 hour, followed by immunohistochemistry and DAPI counterstaining [1]

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Storage: Store sealed in original packaging in a cool, dry place away from light

After a 30-minute incubation on ice, blood samples were collected at various time points post-intravenous 10 mg/kg dose or intraperitoneal 20 mg/kg dose administration of Dihexa dissolved in 75% DMSO

The microsomes were incubated with 500 M solutions of Dihexa, piroxicam, verapamil, and 7-ethoxycoumarin, as controls for low, moderate, and highly metabolized compounds, respectively, in 0.1 M Tris buffer with an NADPH-regenerating system at 37C

Store in a cool, dry place away from direct sunlight