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Once frozen and thawed, the solution appears normal but has lost therapeutic activity and must be discarded

Nitric oxide relaxes the smooth muscles and causes vasodilation and an increase in the blood flow

Therefore, measuring GSH levels to determine the availability of cysteine as the limiting step must be considered in future studies

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Among these factors, the dynamic balance of iron and the abnormality of lipid peroxidation are the most important to induce ferroptosis, while the functional state of System X c and the activity level of GPX4 are the key pathways to regulate ferroptosis