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The detection principle is based on the cascade reaction of GR catalyzing the reduction of GSSG to GSH: GR first uses NADPH to reduce GSSG to GSH, and then the newly generated GSH reacts with the substrate DTNB to form yellow TNB and GSSG

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(1997) Structure, catalytic mechanism, and evolution of the glutathione transferases

Selenium for Male Fertility A trial reported that selenium with vitamin E improved semen parameters in a portion of participants, and some spontaneous pregnancies occurred

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GLUTATHIONE Glutathione is a powerful antioxidant