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Pre-incubation and dilution assay GSTO1 (2 g) was incubated with inhibitor at a concentration of 10 IC 50 in a 10 l reaction volume for 30 min at 37 C

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The total radical-trapping antioxidant parameter (TRAP) assay quantifies the capacity of antioxidants to delay fluorescence decay in a probe (such as R-phycoerythrin or dichlorofluorescein) under controlled peroxidative conditions initiated by AAPH-generated ROO

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