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Parthenogenetic activation After maturation culture, approximately 30 oocytes with the first polar body were collected and treated in preequilibrated Ca 2+ -free (CZB) medium that contained 10 mM SrCl 2 (A500908

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Injection site rotation: Move sites week to week (abdomen, thighs, upper arms), keeping each shot at least 1 inch from the last to limit irritation and prevent lipohypertrophy [15]