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Controls included blots that were not overlaid with the bait but incubated with primary and secondary antibodies or secondary antibodies alone

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It was called vitamin BT in the mid-century, around the 1950s, by the German scientist Fraenkel and the American Blewett

The resulting salt was coupled with another dipeptide 5 using a combination of N , N -dicyclohexylcarbodiimide (DCC) and N -hydroxynorbornene-2,3-dicarboximide (HONb) to yield a tetrapeptide product 7