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For those training fasted (common during morning sessions in India), the post-workout window becomes even more critical because MPS is already compromised before training begins

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EdU (5-Ethynyl-2-Deoxyuridine) Labeling To label mitotically cycling cells, worms were incubated with rocking in 0.2 mL M9 buffer (3 g KH 2 PO 4 , 6 g Na 2 HPO 4 , 5 g NaCl, 1 mL 1M MgSO 4 , H 2 O to 1 L) containing 0.1% Tween 20 and 1 mM EdU for 30 min at 20C