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Representative tissue specimens were collected, dehydrated and embedded in paraffin blocks, sectioned at 4 m, and stained with hematoxylin-eosin according to the following automated Sakura Tissue-Tek DRS 2000 Slide Stainer protocol ( accessed on 19 October 2021): rehydration in distilled water, staining with hematoxylin, washing in running tap water, differentiation with 70% alcohol, staining with eosin, dehydration, clearing, and mounting

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Continue at the effective dose identified during optimization