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Inject into the middle front or outer portion of your thigh, avoiding areas near the knee and groin

Apoptotic signaling, another important determinant of muscle integrity under toxic stress, was also evaluated

The animals were then perfused with cold saline to free them of residual blood, after which liver and other tissues were collected immediately, weighed, quickly diced, and frozen in liquid nitrogen at 80 C

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You can obviously park it till later in the protocol and try again, but you can also use it topically