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The second BglII site at position 7107 in pcDNA3-GFP11(x7)-Actin was removed by site directed mutagenesis using Fwd 5- TGACGTCGACGGATCGGGAAATATCCCGATCCC-3 and Rev 5- GGGATCGGGATATTTCCCGATCCGTCGACGTCA-3 primers with the Quick-Change Lightning kit (Agilent) prior to in frame cloning of human VAPB using T4 DNA ligase (Promega)

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The presence of multiple binding sites allows for specialization, as observed in our FRET experiments

Late circumcision does not seem to have the same preventive effect (152)

Novo Nordisk-CAS Research Fund grant NNCAS-20171-CC (DY) and SA-SIBS Scholarship Program (DY)

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