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Islets were purified using an Optiprep gradient (Nycomed, Oslo, Norway) as previously described 81 and cultured for 18 h in a 37 C in a 5% CO 2 incubator in Roswell Park Memorial Institute (RPMI) medium supplemented with 10% heat-inactivated fetal bovine serum before use in experiments

In addition to being able to act as a physical barrier, there are high concentrations of antioxidants within the ELF that act to detoxify exogenous or endogenous oxidants [4, 10, 11]

NAC has also been shown to help women with endometriosis by reducing pain, shrinking endometriomas, and improving fertility outcomes

This unique sex difference was subsequently confirmed in two follow-up studies, though with more modest reported improvements in female lifespan 7,8 , while a life shortening effect was observed in males 8

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