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We verify the physiological relevance of this unique closed conformation by disulfide crosslinking experiments

Alexiev B, Chou P, Jennings L

Example 4 Step 1: weighing injection water with the prescription amount of about 30 percent, cooling to 20-30 , and filling high-purity nitrogen into the injection water to replace oxygen in the solution until the oxygen content in the solution is less than 1.5 ppm

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