The extract was rotary evaporated at 30 C to a volume of 2 ml, acidified to pH 4 with formic acid, and 200 l aliquots were purified using preparative HPLC (Agilent Technologies, 1260 Infinity) over a Luna 5 m phenyl-hexyl 100 (250 10 mm) column and a 15 min linear gradient from 0% acetonitrile in H 2 O to 16% acetonitrile in H 2 O at 4 ml min 1 flow
The brokerage estimates Indias weight-loss market at about $500 million, which could rise to $1 billion with the right pricing, adoption and government incentives. Generic drugmakers are also experimenting with a range of delivery formats, including single-use pre-filled syringes, single-shot pen injectors, vials, and reusable pens with adjustable dosing, the people familiar with the matter said
This is because the purpose is to harvest the fat cells and not destroy them as with ultrasound and laser liposuction procedures
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