Protein bands were visualized using Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, Billerica, MA, USA) and a ChemiDoc MP Imaging System with Image Lab Touch Software Version 1.0 (Bio-Rad Laboratories, Hercules, CA, USA)
The complexes were embedded in a bilayer composed of 195200 POPC lipids and solvated with 0.15 M NaCl in explicitly TIP3P waters using CHARMM-GUI Membrane Builder v3.2.2 54
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| Placebo | 2.5 | 25 | 10 | 3 | 1 | 2
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Peptides are targeted signals: they send instructions to specific cells, tissues, or hormones rather than acting globally