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Islets were then lysed using acidic ethanol (75% ethanol, 15 mM HCl), sonicated 3X 10 seconds and centrifuged at 1,000 rpm for 3 minutes, and supernatants collected to assess internal insulin content and stored at -20C

So, I dont think any of thats necessary

(iii) Develop more accurate technologies (e.g., smartwatch tools)

In contrast, GLP-1 agonists like Wegovy and Ozempic are long-lasting and act directly on the GLP-1 pathway, making them more potent than probiotics