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RNA extraction and real-time PCR Total RNA was isolated using the RNeasy Mini kit (Qiagen) following the manufacturer's protocol

[10] Medical uses for Semax include treatment of stroke, transient ischemic attack, memory and cognitive disorders, peptic ulcers, optic nerve disease, and to boost the immune system

This interference inhibits the IFN-I signaling cascade in macrophages, ultimately suppressing the host immune response (Sun et al., 2023a)

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To date, studies have mainly focused on its role in tumors and various neurological disorders, including epilepsy