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Functional Improvement Phase Common Experiences Noticeably faster recovery from exercise More stable energy throughout the day Reduced afternoon fatigue and brain fog Improved mood and stress resilience Water retention typically normalizes What to Focus On Increase training intensity if recovery supports it Track workout performance for objective measurement Note any changes in appetite or hunger patterns Week 5-6: The Turning Point Around the five to six week mark, many users experience what researchers describe as the inflection point

A double-blind testing of the X39 patch found it increases GHK-Cu levels

10.1016/j.phymed.2024.155636 99 DavisA

Assuming you followed the above advice and used an appropriate binding buffer and your protein isnt huge, then a likely reason for this is that the expression yield is much lower than you expected

Your liver naturally produces it, but levels can drop due to stress, poor diet, and environmental toxins

It also mimics Glial Cell Line-Derived Neurotrophic Factor (GDNF)