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Both of these sites comprise large apolar cavities with defined polar features ( The first high-affinity, de novo , peptide-based ligands were discovered using phage display ( The concept of using fatty acids as albumin tags to prolong the duration of action of peptides and proteins has been extensively investigated by Novo Nordisk and others

Samples were centrifuged at 5000 g for 30 seconds, and bead pellets washed 3 in 500 L wash buffer (50 mM Tris-HCl, 150 mM NaCl, pH 7.4) before storage at 80 C until further analysis

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