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Western immunoblotting was conducted to examine protein expression related to key signaling pathways and cellular stress responses

The dose is further escalated (titrated) by increasing by 2.5 mg every 4 weeks until reaching the maintenance dosage

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Methods: We use mathematical models for hepatic glutathione metabolism, including one-carbon metabolism and acetaminophen detoxification, to investigate how the activation of certain enzymes by estradiol leads to dramatic changes in reaction velocities and metabolite concentrations