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Confocal microscopy and immunofluorescence Cells were plated at a density of 4 10 3 cells/well on 96-well plates (Perkin Elmer Cell Carrier Ultra Viewer)

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Curcumin targets the Keap1-Nrf2 pathway by modifying Keap1s thiol groups, destabilizing the Keap1-Nrf2 complex, and blocking Nrf2 ubiquitination

Supplementary Table 1 (download XLSX Tab 1: Mutations found in individual clones in first generation evolution