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Don't store it on the refrigerator door, where temperatures fluctuate the most

But when oxidative stress is high (excess reactive oxygen species), it can be used up more quickly.[ref] Studies show that Vitamin C increases nitric oxide through increasing BH4 bioavailability.[ref] Curcumin for NOS3

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Sterile water lacks preservatives and is single-use, making bacteriostatic water preferable when repeated vial access is required

For a 5 mg vial with a target dose of 250-500 mcg twice daily, adding 2 mL of bacteriostatic water creates a 2.5 mg/mL concentration

Some researchers prefer morning to avoid potential nausea interfering with sleep