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The different organs including heart, liver, spleen, lung, and kidney were collected, washed and then homogenized in 1.0 mL of lysis buffer with superfine homogenizer

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Most protocols include periodic breaks or cycling strategies to maintain pituitary responsiveness

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Vitamin E (-tocopherol) is found in the cell membrane of the oocyte and is evidently the first line of defense recruited to disrupt the chain of fatty acid peroxidation protecting the oocyte from further oxidative damage [92]