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So if you know your protein of interest is happy in another buffer, you can likely use that for the binding buffer

10.1097/TA.0b013e31822a3254 106 Zivanovic-PosilovicG.BalenovicD.BarisicI.StrinicD.StambolijaV.UdovicicM.et al (2016)

Simulation system equilibration and energy minimization Following preparation, the systems were energy minimized with an energy tolerance of 100 kj/mol and equilibrated in six sequential steps involving loosening of positional restraints on lipid atoms, protein backbone atoms, and protein side chain atoms (equilibration parameters in Extended Data Table 2)

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In other words, these substances are potentially dangerous, possibly experimental, but tempting to athletes seeking an extra edge

Sources of drift include stimulation regimens, progesterone exposure timing, sampling windows, media formulation, incubator conditions, and operator-dependent handling [462, 470]