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Then, the cells were subjected to immunofluorescence staining for -SMA, desmin, GFAP, and vimentin (Supplementary Table 2) by an overnight incubation with primary antibodies at 4 C, followed by a 1-h incubation with secondary antibody (1:500, Molecular Probes) after a wash step

A role for calcium-calmodulin in regulating nitric oxide production during skeletal muscle satellite cell activation

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Moreover, SiO 2 NPs exhibited the highest elicitor efficacy (2.23-fold), followed by MWCNTs (1.69-fold) and ZnONPs (1.11-fold)

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Discontinuous gradient method: Dilute OptiPrep with 0.25 M sucrose, 6 mM EDTA, 60 mM Tris-HCl, pH 7.4 to produce 14%, 25% and 55% (w/v) iodixanol