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5 In addition, high-intensity exercise also results in an increased production of lactic acid, which can further contribute to oxidative stress by causing a decrease in pH levels in the muscle tissues

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Biochemical analyses Transfected N2a cells were lysed with ice-cold Lysis buffer (20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 5 mM MgCl 2 , 2 mM EDTA, 1% Triton X-100, 0.1% SDS, and 10% glycerol) supplemented with a protease inhibitor cocktail (Roche)

One reason may have to do with how trials measure the hormones effectiveness

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