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On balance, any improvements in plasma homocysteine from moderate coffee consumption (approximately 150ml per day of the filtered preparation) may be due to a synergy of the beneficial polyphenols ingested at optimal dietary levels acting on biochemical pathways that are yet to be elucidated

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Quantification of GSH We used the Arbor Assay DetectX Glutathione Detection Kit (#K006, Arbor Assay, Ann Arbor, MI, USA), with which we have experience (e.g., ref