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d , Recombinant Flag-CAMK2 WT or Flag-CAMK2-KD purified from 293T cells (100 ng) was incubated with bacterially purified His-PSAT1 (1000 ng) in 40 l of kinase buffer (10 mM HEPES (pH 7.2), 1 mM EGTA, 5 mM MgCl 2 and 2 mM CaCl 2 ) and then incubated with 0.5 mM normal ATP at 30 C for 30 min and then subjected to SDSPAGE and immunoblotting

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