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ACSS2 KO cells used glucose similarly to their WT counterparts and labeling from acetate was completely ablated, showing that ACSS2 is the primary acyl-CoA synthetase that mediates synthesis of acetyl-CoA from acetate in these cells (Figure 1D)

And uh, before I begin, just as a reminder, this is, uh, not a substitute for professional medical advice, diagnosis, or treatment