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Samples were then soaked in a 30% sucrose solution for 68 h at 4C, finally included in freezing resin (OCT, Killik, Bio-Optica, Milan, Italy) and cryostat-cut in longitudinal sections (10- m thick)

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Some patients have a body mass index in the obesity range, while others are overweight with at least one weight-related health condition, such as high cholesterol, elevated blood sugar, heart disease risk factors, sleep apnea, or other weight-related medical problems