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It is possible that the acenocoumarol dosage may need to be adjusted

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Biotinylation of E3 ligase modules for BLI binding assays To prepare site-specifically biotinylated versions of WDR26 CTLH and YPEL5WDR26 CTLH for BLI assays, biotin was conjugated to the AviTag attached to the WDR26 CTLH C terminus in the BirA ligase-catalyzed reaction

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Eluates were separated on a 515% gradient SDS-PAGE gel and the bands revealed through colloidal Coomassie staining