Isolation and culture of pancreatic islets Mice were sacrificed by cervical dislocation and the pancreas was excised after infusing the animal in the common bile duct with 34 mL of Collagenase P solution (13.5 U/mL) derived from Clostridium histolyticum mixed with HBSS (1:14) following the procedure reported by Carter et al
Thermal sensitivity measurement (Hargreaves test) On the day of the behavioral experiment, the mice were initially given a one-hour period to acclimate to the plexiglass chambers, which measured 6.5 6.5 6 cm, on the Hargreaves Apparatus (IITC Life Science Inc., CA, USA)
D., Latsko, M
Common adverse effects include nausea, vomiting, diarrhoea, and constipation, which typically diminish over time
The 2025 NEJM trial comparing tirzepatide directly against semaglutide showed 20.2% versus 13.7% weight loss over the same treatment period
In our study, treatment with semaglutide, a long-acting GLP-1RA, effectively mitigated core symptoms of BBS, including reduced food intake and decreased body weight, and improved glucose tolerance and neurobehavioral function
