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In the negative control, normal rabbit IgG (ab172730, Abcam, Cambridge, UK) diluted to 4 g/mL in 1% rabbit serum in PBS was used instead of the primary antibodies

Clinical trials would need to assess both hemodynamic parameters and clinical outcomes including walking distance, pain scores, and amputation rates to establish therapeutic benefit

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Note what you eat, portion sizes, and any subsequent bloating symptoms

Consider a fiber supplement

Storage discipline is the difference between a protocol that works and one that wastes time and money while delivering nothing