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After treating HaCaT with 580 M CuCl 2 or Cu(OAc) 2 , the expression of IL1A, IL8, HSPA1A and FOSL1 were significantly higher than that in the control group and ranged from a 844 fold change

However, in clinical practice, troublesome GERD symptoms persist in 20 30% of patients despite daily treatment with a proton pomp inhibitor (PPI)

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Biotinylated goat anti-mouse IgG or goat anti-rabbit IgG (Vector Labs, BA-9200 1:200 and BA-1000 1:200, respectively) secondary antibodies were added in blocking buffer and incubated for 1 h at room temperature

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