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For viability, 7-aminoactinomycin D (7AAD) (Thermo fisher A1310) or Zombie NIR (BioLegend 423106) diluted 1:3000 was stained concurrently with antibodies in all flow cytometry assays

130 , 796797 (2008) Ralat, L.A., Manevich, Y., Fisher, A.B., Colman, R.F.: Direct evidence for the formation of a complex between 1-cysteine peroxiredoxin and glutathione S -transferase with activity changes in both enzymes

Overall, regular follow-up care is crucial for the long-term management of vitamin B12 deficiency and to promote overall health and well-being

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In the autotrophic tissues, the upregulated antioxidant enzyme activities led to reduced levels of H 2 O 2 and MDA levels under NaCl stress

Finally, the cells were washed with PBS for 3 times and imaged using a laser confocal microscope (Excitation: 488 nm)