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Educating the patient about insulin therapy helps alleviate any fears or misconceptions they may have and promotes acceptance and adherence to the prescribed treatment regimen

[29] For example, a 2012 study published in Nutrients found that people with B12 deficiency can have elevated levels of inflammatory markers like TNF- and resistin

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RNA isolation and qRTPCR analysis Total RNA was extracted using TRIzol (Takara Bio, Tokyo, Japan) and quantitative reverse transcription polymerase chain reaction (qRTPCR) was performed using Hieff qPCR SYBR Green Master Mix (Vazyme, Nanjing, China) according to the manufacturers instructions

Recurrence has not been reported

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