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For co-immunoprecipitation assay, the expression vector of FLAG-PGRMC1 or an empty vector into HCT116 by using a transfection reagent Lipofectamine 2000 (Invitrogen)

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Then, the FDA declared in late 2024 that tirzepatide was no longer in shortage, and it determined that semaglutide was no longer in shortage in early 2025

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