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The finding was further supported by c-met-binding experiments, in which HGF was pre-treated with GSSSG and LASSS at the same molar ratio 1:4000 at which the satellite-cell activation index was rescued to a level comparable to that of non-nitrated regular HGF, as shown in Figure 1d, followed by exposure to peroxynitrite (1:2000) and evaluated for c-met-binding by a sandwich ELISA-like assay on recombinant c-met-Fc chimera (Figure 1e, left column

Birari L, Wagh S, Patil KR, Mahajan UB, Unger B, Belemkar S, et al

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Picomolar to nanomolar activity suggests high receptor affinity and potent biological signalling, which is encouraging for topical delivery where only a fraction of applied compound reaches the follicle

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