After centrifugation, 10 l of the supernatant were filtered through a Microcon YM-10 centrifugal filter device (Amicon), and analyzed by reversed-phase high-performance liquid chromatography on a C18 column (Atlantis 5 m, 4,6200 mm, Waters) as previously described [18]
Perspectives in GLP-1 research: New targets, new receptors
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