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Cells were washed once more with 1 PBS and then stained with 02 g L 1 TRITC-phalloidin in 3% BSA-PBS for 30 min

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Statements Author contributions The author searched the available literature on the topic using PubMed, Embase, clincal.trials.gov and is the sole independent author of this manuscript and its content

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How to use it For most use scenarios that basically aim to help reduce portion size, the recommended approach is to take Calocurb an hour before a meal, with a large glass of water