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In this approach, NO is generated from the donor compound 3-(2-hydroxy-1-methyl-ethyl-2-nitrosohydrazino)-N-methyl-1-propanamine, which is oxidized to NO2 by the target compound 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (carboxy-PTIO)

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The LC-MS/MS analysis was performed using a Sciex QTRAP 6500 + - MS connected to a Sciex AD UHPLC

This is the formulation most similar to branded injectable products