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Vily-Petit J, Soty M, Silva M, Micoud M, Bron C, Gurin-Deremaux L, Mithieux G

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Acta Pharm Sin B 13(12):46674687 Firoozi D et al (2024) Effects of short-chain fatty acid-butyrate supplementation on expression of circadian-clock genes, sleep quality, and inflammation in patients with active ulcerative colitis: a double-blind randomized controlled trial

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Some researchers report success with single-freeze protocols (reconstituting, aliquoting into single-dose volumes, and freezing once), but this is not standard practice and may affect potency unpredictably