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7) Liver Dysfunction In a study on over 1,600 adults, high TMAO levels were associated with more severe liver damage and non-alcoholic fatty liver disease [33]

Notably, these effects are more effective for chronic rather than acute pain and are achieved without the development of tolerance [31, 36, 38, 40,41,42]

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The next day, the wells were blocked for 1 h (4% bovine serum albumin (BSA), 5% sucrose in phosphate-buffered saline (PBS)), and then 100 L of sample was added and incubated for 12 h