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de Almeida JM, Theodoro LH, Bosco AF, Nagata MJ, Oshiiwa M, Garcia VG

After 15 min of incubation at room temperature in the dark, cells were washed, resuspended in the binding buffer, and DAPI was added immediately before analysis on a Fortessa X-20 Cell Analyzer (BD Biosciences)

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Currently, prodrugs under research comprise TLK286, purine analogs, 77 sulfonamides, 78 and brostallicin